PROSITE documentation PDOC50890
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{PDOC50890} {PS50890; PUA} {BEGIN} ********************** * PUA domain profile * ********************** The PUA domain, which has been named after PseudoUridine synthase and Archaeosine-specific transglycosylase, is a small, compact domain that consists of 78-83 amino acid residues. The PUA domain is widely conserved in eukaryotic and archaeal RNA modification enzymes and is found in: - Archaeal and eukaryotic pseudouridine synthases. - Archaeosine tRNA-guanine transglycosylases. - A family of predicted ATPases that may be involved in RNA modification. - A family of predicted archaeal and bacterial rRNA methylases. - A conserved family of putative novel eukaryotic translation factors. - Glutamate 5-kinases (EC 2.7.2.11). The PUA domain can be found alone or in association with other domains, such as the glutamate kinase domain (see <PDOC00701>), the pseudouridine synthase catalytic domain, the phosphoadenosine phosphosulfate (PAPS) reductase domain, the methylase domain, the TGT domain, or the SUI1 domain (see <PDOC00862 >). The PUA domain is supposed to bind to RNA molecules with complex folded structures [1]. The PUA domain contains highly conserved motifs that center around stretches of hydrophobic residues. The domain also contains two highly conserved positions occupied by small amino acids (primarily glycines) [1]. The resolution of the crystal structure of archaeosine tRNA-guanine transglycosylase from Pyrococcus horikoshii has shown that the PUA domain consists of two alpha-helices and six beta-strands, and folds into a beta- sandwich structure similar to the oligonucleotide-binding (OB)-fold (see <PDB:1IQ8>) [2]. The profile we developed covers the entire PUA domain. -Sequences known to belong to this class detected by the profile: ALL. -Other sequence(s) detected in Swiss-Prot: NONE. -Last update: November 2002 / First entry. [ 1] Aravind L., Koonin E.V. "Novel predicted RNA-binding domains associated with the translation machinery." J. Mol. Evol. 48:291-302(1999). PubMed=10093218 [ 2] Ishitani R., Nureki O., Fukai S., Kijimoto T., Nameki N., Watanabe M., Kondo H., Sekine M., Okada N., Nishimura S., Yokoyama S. "Crystal structure of archaeosine tRNA-guanine transglycosylase." J. Mol. Biol. 318:665-677(2002). PubMed=12054814; DOI=10.1016/S0022-2836(02)00090-6 -------------------------------------------------------------------------------- PROSITE is copyrighted by the SIB Swiss Institute of Bioinformatics and distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives (CC BY-NC-ND 4.0) License, see https://prosite.expasy.org/prosite_license.html -------------------------------------------------------------------------------- {END}